DIA-HВV – Enzyme immunoassay test system for the detection of hepatitis B virus surface antigen (HBsAg)

Principle of the method: one-stage procedure based on «sandwich»-ELISA Clinical materials: human serum or plasma Immunosorbent – monoclonal antibodies to HBsAg Conjugate – monoclonal antibodies to HBsAg conjugated with horseradish peroxidase Packing configuration: strip (lockwell) or monolith microplate 96 or 192 tests TMB chromogen Incubation time: 2 hours Shelf life: 14 months Sensitivity: 0,3 ng/ml HBsAg – for kits packed with OPD 0,2 ng/ml HBsAg – for kits packed with TMB Specificity – 99.2 % (8869 serum samples from blood donors)	Assay principle
		wells coated with monoclonal antibodies to HBsAg
		adding of controls, sera and conjugate incubation for 120 minutes, 37 °С
		adding of chromogen incubation for 30 minutes at 18-25°С reaction termination result reading

DIA-С-HВV –  Enzyme immunoassay test system for the confirming of hepatitis B virus surface antigen (HBsAg)

Principle of the method: verification of results based on neutralization- binding of conjugate with HBsAg by antibodies composing of neutralization component Clinical materials: human serum or plasma Using high specific monoclonal antibodies to HBsAg composing of immunosorbent, neutralization component and peroxidase conjugate Packing configuration: strip microplate 96 tests TMB chromogen Incubation time: 2,5 hours Shelf life: 12 months	Assay principle
		wells coated with monoclonal antibodies to HBsAg
		adding of sera, conjugate and neutralization component incubation for 120 minutes, 37°С
		adding of chromogen incubation for 30 minutes, 18-25°С reaction termination result reading

Dynamics of serological markers of HBV

Relative concentration

Time after infection

DIA-HBcore – Enzyme immunoassay test system for the detection of IgG and IgM-antibodies to the hepatitis B virus core protein

Principle of the method: verification of results based on neutralization- binding of conjugate with HBsAg by antibodies composing of neutralization component Clinical materials: human serum or plasma Using high specific monoclonal antibodies to HBsAg composing of immunosorbent, neutralization component and peroxidase conjugate Packing configuration: strip microplate 96 tests TMB chromogen Incubation time: 2,5 hours Shelf life: 12 months	Assay principle
		wells coated with recombinant HBsAg antigen
		adding of sera incubation for 60 minutes, 37°С
		adding of conjugate incubation for 30 minutes, 37°С
		adding of chromogen incubation for 30 minutes, 18-25°С reaction termination result reading