DIAGNOSTICS OF CLAMIDIOSIS

DIA-Chlamydia – Enzyme immunoassay for the detection of IgG and IgA classes antibodies to the Chlamydia trachomatis Principle of the method: two-stage procedure based on indirect solid ELISA Clinical material: human serum or plasma Detection of specific antibodies of IgG and/or IgA types Absence of false-positive reactions caused by cross-reactions between different species of Chlamydia Immunosorbent – recombinant antigen is an analogue of main protein of the surface membrane of Chlamydia trachomatis (MOMP) «Conjugate IgG» is monoclonal antibodies to human IgG linked with horseradish peroxidase «Conjugate IgА» is monoclonal antibodies to human IgA linked with horseradish peroxidase Packing configuration: strip (lockwell) microplate 96 or 192 tests TMB chromogen Supplied reagents in the kit are enough for the performance of all tests (96 or 192) for revealing of IgG or/and IgA Incubation time – 2 hours Shelf life: 12 months Sensitivity and specificity – 100% on serum panels (МBU, Russia), which contain and not contain IgG and IgA antibodies to Chlamydia trachomatis. Assay principle wells coated with recombinant protein МОМР (Major Outer Membrane Protein) adding of sera incubation for 60 minutes, 37°С adding of conjugate incubation for 30 minutes, 37°С adding of chromogen incubation for 30 minutes, 18-25°С reaction termination result reading Primary infecting Chronic infection time after infection time after infection Interpretation of results of IgG/IgA detection Presence of antibodies to Chlamydia trachomatis Result interpretation IgG IgA Negative Negative Negative, or the samples contains very low antibody titer * Negative Indetermined or positive Possibility of early phase of infection* Indetermined or positive Negative Past infection Positive Positive Acute or chronic infection